Application

Suggested dilution for most applications: 1:50-1:200 for most applications.Optimal working dilutions must be determined by the end user.

Research CategorySecondary & Control Antibodies

Research Sub CategorySecondary Antibodies Adsorbed for Dual Labeling

Goat anti-Mouse IgG Antibody, FITC conjugate, Species Adsorbed detects level of Mouse IgG & has been published & validated for use in IF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6, with .01% Thimerosal and .05% Sodium Azide.

RECONSTITUTION:

Reconstitute with 0.45 mg/mL with sterile distilled water.

Specificity

Mouse IgG (H+L) (absorbed for minimal cross-reactivity to Rat, Human, Bovine and Horse Serum Proteins)

FLUOROPHORE/PROTEIN ABSORBANCE RATIO:

Fluorescein Isothiocyanate 10.0 ug/mg; 3.8 moles FITC per IgG

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.